Journal: Thrombosis and haemostasis

Article Title: Endotoxaemia-augmented murine venous thrombosis is dependent on TLR-4 and ICAM-1, and potentiated by neutropenia *

doi: 10.1160/TH16-03-0218

Figure Lengend Snippet: WT mice pre-treated with i. p. injection of LPS 10 mg/kg or equivalent volume of vehicle (control) underwent ligation and thrombi were harvested at 2 h post thrombosis. Platelets were quantified by CD41 western immunohistochemistry (n=6–10, p=0.0010) (A). Representative photomicrographs of CD41 staining of thrombi of saline treated mice (B) and LPS-treated mice (C) shown at 10x. Neutrophils were counted in the thrombi of control and LPS treated mice utilising Ly6G antibody (n=6, *p=0.003) (D). Representative histology of thrombus stained in IVC is shown in panels E (control) and F (LPS treatment) stained with Ly6G antibody at 10x. PMN markers cathepsin G and MPO were measured via western blot (n=5–6, *p<0.05) (G-H). Circulating factor XIII was measured by western blot (n=4–7, p=0.045) (I).

Article Snippet: After blocking non-specific sites with species-specific serum, sections were stained for activated PMNs by Ly6G (BD Pharmingen, San Diego, CA, USA), ICAM-1(R&D Systems, Minneapolis, MN, USA), TLR4 (Thermo-Fisher Scientific, Invitrogen, Waltham, MA, USA)staining.

Techniques: Injection, Ligation, Western Blot, Immunohistochemistry, Staining

Journal: Thrombosis and haemostasis

Article Title: Endotoxaemia-augmented murine venous thrombosis is dependent on TLR-4 and ICAM-1, and potentiated by neutropenia *

doi: 10.1160/TH16-03-0218

Figure Lengend Snippet: A select group of mice underwent PMN depletion via a single IP injection (250 ug) of purified rat anti-mouse Ly6G clone antibody or control rat IgG2b. LPS-treated mice undergoing no treatment (true control), IgG (control antibody) or Ly6G (neutrophil depletion) underwent VT and with subsequent measurement of PMNs (n=4–10, p<0.05) (A). Thrombus size was measured 2 h following induction of VT (n=5–6, *p<0.05) (B). Enzymes involved in fibrinolysis, PAI-1 (n=4–6, *p<0.05) (C) and uPA (n=4–6 *p<0.05) (D), were measured in vein wall in thrombosed animals via western blot. PAI-1 =plasminogen activator inhibitor, uPA=urokinase plasminogen activator.

Article Snippet: After blocking non-specific sites with species-specific serum, sections were stained for activated PMNs by Ly6G (BD Pharmingen, San Diego, CA, USA), ICAM-1(R&D Systems, Minneapolis, MN, USA), TLR4 (Thermo-Fisher Scientific, Invitrogen, Waltham, MA, USA)staining.

Techniques: Injection, Purification, Western Blot